Journal: Frontiers in Endocrinology

Article Title: miR-29a/b 1 Regulates the Luteinizing Hormone Secretion and Affects Mouse Ovulation

doi: 10.3389/fendo.2021.636220

Figure Lengend Snippet: Central mechanism in miR-29a/b 1 KO mice. (A, B) GnRHR immunoreactivity in pituitary of wild-type and miR-29a/b 1 KO females. The receptor was not detectable on the plasma membrane of control. (immunohistochemical: wild-type: 13.43 ± 0.7927, miR-29a/b 1 KO: 25.47 ± 0.534, p=0.0249; immunofluorescence: wild-type: 37.79 ± 1.858, miR-29a/b 1 KO: 56.64 ± 2.767, p=0.0045 , n=3). (C) Serum LH and FSH levels in miR-29a/b 1 KO females and controls following ovariectomy (OVX) and sham-operated controls (Sham). (LH: wild-type: p=0.0401 , miR-29a/b 1 KO: p=0.9249 ; FSH: wild-type: p=0.0016 , miR-29a/b 1 KO: p=0.0185 , n=6). (D) Expression of Kiss1 and Gnrh1 in hypothalamus (Gnrh1: wild-type: 1 ± 0.1912, miR-29a/b 1 KO: 0.7287 ± 0.06234, p = 0.1874 , Kiss1: wild-type: 1 ± 0.1305, miR-29a/b 1 KO: 0.8142 ± 0.0757, p=0.8405 , n=15). (E) Normal distribution of GnRH neurons in miR-29a/b 1 KO mice compared to control littermates. OVLT, organum vasculosum of the lamina terminalis. Scale bars, 200μm. (wild-type: 9.827 ± 1.547, miR-29a/b 1 KO: 8.597 ± 0.8466, p=0.5238 , n=3). * p < 0.05, ** p < 0.01 and **** p < 0.0001.

Article Snippet: After permeabilization, the sections were washed three times in PBST, and blocked with 5% normal donkey serum in PBS for 1h at room temperature, then were incubated with mouse anti-Lutropin beta antibody (1:1000, SANTA CRUZ, sc-373941), rabbit anti-GnRH1 (1:500, Immunostar, PA1-121) or rabbit anti-GnRHR antibody ( ) (1:100, Proteintech, 19950-1-AP) overnight at 4°C, followed by staining with Alexa Fluor 647-conjugated donkey anti-mouse antibody (Invitrogen Molecular Probes) or Alexa Fluor 594-conjugated donkey anti-rabbit (Invitrogen Molecular Probes) antibody and DAPI dye to stain nuclei.

Techniques: Immunohistochemical staining, Immunofluorescence, Expressing

Journal: Scientific Reports

Article Title: Increased Uterine NK cell numbers and perforin expression during the implantation phase in IVF Cycles with GnRH Antagonist Protocol

doi: 10.1038/srep39912

Figure Lengend Snippet: Representative images and quantification of FasL ( A , E ), perforin ( B , F ), uNK cells ( C , G ), and GnRHR ( D , H ) of four groups assayed by Western blot. All data are mean values ± standard deviation, and considered statistically significant at P < 0.05. Cropped gels and blots are displayed, and full-length gels and blots are included in the .

Article Snippet: The following primary and secondary antibodies were used: human NCAM-1/CD56 affinity-purified polyclonal antibodies (AF2408, R&D), mouse anti-human GnRHR monoclonal antibodies (ab24095, Abcam), human anti-FasL/TNFSF6 affinity-purified polyclonal antibodies (AF126, R&D), and rabbit anti-perforin polyclonal IgG (SC9105, Santa Cruz).

Techniques: Western Blot, Standard Deviation

Journal: Scientific Reports

Article Title: Increased Uterine NK cell numbers and perforin expression during the implantation phase in IVF Cycles with GnRH Antagonist Protocol

doi: 10.1038/srep39912

Figure Lengend Snippet: Representative images of FasL ( A ), perforin ( B ), uNK cells ( C ), and GnRHR ( D ) of four groups using immunohistochemical assay.

Article Snippet: The following primary and secondary antibodies were used: human NCAM-1/CD56 affinity-purified polyclonal antibodies (AF2408, R&D), mouse anti-human GnRHR monoclonal antibodies (ab24095, Abcam), human anti-FasL/TNFSF6 affinity-purified polyclonal antibodies (AF126, R&D), and rabbit anti-perforin polyclonal IgG (SC9105, Santa Cruz).

Techniques: Immunohistochemical staining